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Fifth Annual
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Characterization of the in vitro Antioxidant Activity of Flavonoids Extracted from Artemisia tridentata ssp. tridentata

Author(s): Erick Valenzuela

Presentation: poster

Sagebrush of the species Artemisia tridentata produces polyphenolic compounds known as flavonoids. Flavonoids are known for their ability to scavenge free radicals, thereby acting as strong antioxidants. Epidemiological studies have also shown that flavonoids play a role in the prevention of degenerative diseases such as cancer and cardiovascular diseases. Flavonoids can be consumed through eating fruits, vegetables, tea, cocoa and wine. Previous studies in our laboratory have shown that there are a wide variety of flavonoids observed after high pressure liquid chromatography (HPLC) of extracts from local Artemisia tridentata ssp. tridentata. This sagebrush extract exhibits antioxidant activity in vitro in a dose dependent manner. The purpose of the current study was to identify which of the flavonoids present in sagebrush extract are responsible for antioxidant activity. The in vitro peroxyradical scavenging capacity (PSC) assay was used to measure the antioxidant activity of different flavonoid compounds extracted from sagebrush separated by reverse-phase HPLC. HPLC fractions were combined into separate pools and concentrated with a TurboVap LV. The antioxidant activity of these sagebrush pools was compared to that of quercetin, a reference flavonoid. HPLC solvents alone had minimal or no antioxidant activity. Results suggest that sagebrush pool B1 exhibits the strongest antioxidant activity.

 

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