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2017 Archive
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Author(s): Micaela Martin, Reed Maggard , Reed Maggard
Presentation: poster
Biomarker utilization allows toxicologists to determine the physiological response that different toxins elicit from animals. Phase I CYP 1A1 detoxification enzymes are recognized as one of the most important pathways utilized by crayfish in eliminating toxins. Ethoxyresorufin-O-deethylase (EROD) activity is indicative of the phase I detoxification enzyme CYP 1A1 and has served as a useful biomarker in aquatic invertebrates. Glutathione S-transferase (GST) is a phase II enzyme that biotransforms toxic compounds allowing excretion. Upregulation of the phase I or II enzymes EROD/MROD or GST is indicative of a biochemical response to xenobiotics. Estrogenic compounds, such as estradiol, have been a growing concern in the realm of toxicology. These estrogenic compounds have been consistently found in treated sewage, soil, and surface water. To determine whether estradiol-17β (E2) modulates the activity of EROD, MROD and GST, Signal crayfish were treated with varying doses of estradiol-17β (E2). The hepatopancreas was then prepped and run through EROD, MROD and GST assays. While there was not a significant response in the females, male detoxification was significantly upregulated compared to females at specific doses.
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