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Saturday May 16, 2020
AUTHORS:
Colt Merges, Kathryn Craig, Daniel Hernandez, Emily Bartlett, Dr. Sara Heggland, and Dr. Thomas Pirtle
FACULTY: Dr. Thomas Pirtle
DEPARTMENT: Biology
With the popularity of electronic cigarettes and personal vaporizers on the rise, the health risks and concerns of inhaling these vapors is widely unknown. Within this inquiry, we examined the effects of different non-nicotine vaporizer liquids on developing osseous tissue in embryonic G. gallus domesticus and treated embryos with 0.1 ml of diluted vaporizer liquids using a windowing method. Each embryo was treated with either vaporized or un-vaporized electronic cigarette vaporizer liquids on day 4 or 7 of embryonic development and were dissected on day 14. We examined the length and girth of the dissected tibias and femurs, which revealed statistically significantly difference in mango strawberry or mocha flavor exposed embryos treated during day 7 in development. Additionally, comparison of bone mineralization through ash weighs of the bones also indicate a significant difference within these same flavor groups listed above. The bone mineralization study revealed increased bone mineralization from heavier ash weights. The differences in bone mineralization and osseous tissue reveal that non-nicotine compounds within the electronic cigarette liquids can alter osseous tissue morphology.
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